Measuring white fat in whole slide images using digital pathology image analysis

White fat measurement

White fat measurement is shown below with a Flagship algorithm that first segments and then individually identifies each fat vacuole. Statistics of area and perimeter per vacuole are counted, and histograms can be output as required. Some results are shown below. In areas where vacuoles are either not easily seen by eye, or where they are very small and merged together, it is best to eliminate these by restricting the vacuole size to be larger.

Original image and markup of fat vacuoles in a histology section — Orginal image of white fat on the left, with the image analysis results on the right

Histograms showing area and perimeter per fat vacuole — Area is graphed on the left, on a per vacuole basis. On the right is perimeter. Both can be useful, depending on the application.

2nd example region showing image analysis results — 2nd example region

Small vacuoles can be eliminated by a vacuole area threshold — In cases like lower right area of this image, the vacuole area threshold can be increased to eliminate problematic areas

Fat vacuoles can be eliminated by adjusting the circularity — Elongated vacuoles can be eliminated by increasing the “circularity” threshold

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White fat measurement

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