Phosphomarkers do not generally represent any additional difficulties in image analysis, although the sample handling and collection is not trivial, and one must take care with interpretation, particularly when working in realworld clinical specimen settings. Below is a phospho-NF-kappa B p65 (Ser 276) antibody from EnVision (Rabbit) on formalin fixed paraffin embedded human colon carcinoma.
While we utilize automated pattern recognition whenever possible, in this case the tumor / stroma had to be delineated manually, with pathologist review. We could also use FACTS with image registration with an antibody for tumor cells as an alternative approach for tumor / stroma identification.
