Measuring alveoli in lung with digital pathology and image analysis

Measuring lung alveoli

For measurement of absolute endpoints in lung tissue, Flagship employs stereological methodology for evaluating surface volume and other pulmonary measurements.

However, two dimensional image analysis can also be useful, particularly when relative measurements between treatment and control groups are the end goal. The ability to identify each alveoli in a lung section, and then to measure statistics on each one is important in several lung-related therapeutic research area including inhalation toxicology and emphysema research. A custom algorithm has been developed where each alveoli is identified, and then perimeter and area can be calculated and the results graphed in a histogram. Some examples are below:

Digital pathology image analysis on a lung section — Lung section. Original image on the left, and markup image on the right, showing where a custom image analysis algorithm has identified each alveoli.

Histograms showing area and perimeter results from lung alveoli finding — Area histogram on the left and perimeter histogram on the right, from each alveoli in the section above

Custom image analysis results using digital pathology on lung section — 2nd zoomed-in region showing individual detection and analysis of lung alveoli

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